Development of an in vivo bioassay to identify Turkish chickpea genotypes resistance to Ditylenchus dipsaci (Kühn, 1857) (Tylenchida: Anguinidae)

Stem and bulb nematode, Ditylenchus dipsaci (Kühn, 1857) (Tylenchida: Anguinidae) is one of the most damaging plant-parasitic nematodes damage legume crops such as chickpea. The aim study was to develop a protocol for in-vivo bioassay investigate D. interaction with Nine accessions wild domesticated Cicer spp. including three reticulatum Ladiz., echinospermum P.H.Davis arietinum L. (Fabales: Fabaceae) were used evaluate chickpea genotype resistance between 2019 2020 at Çukurova University, Nematology laboratory. incubation time inoculum density determined correlate size produced nematode population. It concluded that highest reproduction on carrot discs occurred 20 25°C 45-60 days an initial 100 per disc. Also, 300 growing 16 weeks best practice identify reaction genotypes . These methods provided quick screening studies in against observe information period time, temperature, density.